![]() Find a range of innovative, high-quality Thermo Scientific Pierce products, including the Pierce antibodies, SuperSignal chemiluminescent substrates, Pierce BCA Protein Assays and other protein biology research tools and reagents. Precision Plus Protein Unstained Standards are also an excellent choice for use with stain-free gels and theĪll Precision Plus Protein Standards generate a standard curve with r 2 values >0.99, demonstrating a strong linear relationship between the proteins' log molecular weight and relative migration distance on a gel. Contact our technical support scientists for experienced troubleshooting and product advice: 5345. These protein standards are compatible with Laemmli and neutral pH buffer systems and can be used for accurate molecular weight determination on gels and blots. These standards offer absolute molecular weight accuracy confirmed by mass spectrometry, with a known amount of protein in each band to allow approximation of protein concentration in your sample. Precision Plus Protein Unstained Standards contain the unique Strep-tag affinity peptide, which allows detection and molecular weight determination on western blots. These unstained protein standards include three high-intensity reference bands (25, 50, and 75 kD). These standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD. Precision Plus Protein™ Standards for protein electrophoresis offer unsurpassed band sharpness, accurate MW determination, lot-to-lot consistency, and excellent blotting results. In blotting applications, the ladder can be used to monitor protein transfer and as a reference to estimate the molecular weight of proteins of interest. The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce. It is ideal for monitoring protein separation during SDS-PAGE, confi rming WB transfer on membranes, and estimating protein sizes. Add Tween 20 detergent to the wash buffer to a final concentration of 0.05. Th e Prime-Step Prestained Broad Range Protein Ladder is a three-color protein standard with 10 prestained chromophore- conjugated recombinant proteins covering a wide range of molecular weights, from 6.5 to 270 kDa. Increase the number of washes and/or the volume of buffer used. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color (blue, orange, green, pink) protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and western blotting. Use Thermo Scientific SuperSignal Western Blot Enhancer to reduce background and enhance detection of low-abundance and weakly immunoreactive antigens.
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